Generator
T7+eGFP+Tm

Part:BBa_K193208:Design

Designed by: Nao Nakatani   Group: iGEM09_Tokyo_Tech   (2009-10-14)


TmTHP with promotor for constituitive expression and with EGFP for fusion of Anti-Freeze Protein


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1047
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1047
    Illegal NheI site found at 1065
    Illegal NotI site found at 1077
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1047
    Illegal BamHI site found at 582
    Illegal XhoI site found at 483
    Illegal XhoI site found at 1086
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1047
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1047
    Illegal AgeI site found at 792
    Illegal AgeI site found at 831
    Illegal AgeI site found at 903
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We used biobrick standard assembly.



Source

We used I719005 and K193205.

References